Part:BBa_K4761223:Design

adh6-linker4-ARO10

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2886
  Illegal SpeI site found at 1837
  Illegal PstI site found at 1715
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2886
  Illegal SpeI site found at 1837
  Illegal PstI site found at 1715
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2886
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2886
  Illegal SpeI site found at 1837
  Illegal PstI site found at 1715
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2886
  Illegal SpeI site found at 1837
  Illegal PstI site found at 1715
  Illegal NgoMIV site found at 991
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Stop codon of adh6 deleted for fusion expression.